CBE661 Bioprocess Engineering Lab UITM Assignment Sample Malaysia

CBE661 Bioprocess Engineering Lab is a course that is typically offered at the undergraduate or graduate level in chemical and biochemical engineering programs. The course focuses on providing students with hands-on experience in designing, operating, and optimizing bioprocesses.

The course may involve laboratory experiments where students work with microbial cultures and perform various operations such as sterilization, media preparation, and bioreactor operation. Students may also analyze data generated from experiments and use software tools to simulate and optimize bioprocesses.

Buy Non Plagiarized & Properly Structured Assignment Solution

Order your assignments for CBE661 Bioprocess Engineering Lab course at the last minute!

Malaysiaassignmenthelp.com is a reputable provider of CBE661 Bioprocess Engineering Lab assignment solutions, aimed at assisting students in achieving the best possible grades. Our team of skilled writers and meticulous proofreaders is readily available to deliver tailor-made projects that align with your specific requirements. With our professional assistance, you can trust that your assignments will be completed to the highest standards, ensuring academic success.

In this section, we discuss some assignment briefs. These are:

Assignment Brief 1: Operate shake flask and bioreactor fermentation, prepare culture media, construct a growth curve and calculate mass transfer coefficient in fermentation.

Operating Shake Flask and Bioreactor Fermentation:

Shake Flask Fermentation:

  • Prepare the culture media according to the requirements of the organism to be cultured.
  • Sterilize the media and distribute it into shake flasks.
  • Inoculate the shake flasks with the organism to be cultured.
  • Incubate the shake flasks in a shaking incubator at the appropriate temperature, pH, and aeration conditions for the organism.
  • Monitor the growth of the organism by taking samples at regular intervals and measuring the optical density or other growth parameters.
  • Harvest the cells at the desired time point for downstream applications.

Bioreactor Fermentation:

  • Prepare the culture media according to the requirements of the organism to be cultured.
  • Sterilize the media and the bioreactor vessel.
  • Inoculate the bioreactor with the organism to be cultured.
  • Set the operating conditions for the bioreactor, such as temperature, pH, agitation speed, and aeration rate.
  • Monitor the growth of the organism by taking samples at regular intervals and measuring the optical density or other growth parameters.
  • Control the operating conditions to maintain optimal growth conditions for the organism.
  • Harvest the cells at the desired time point for downstream applications.

Preparing Culture Media:

  • Determine the nutrient requirements of the organism to be cultured.
  • Select appropriate ingredients and prepare the media according to established protocols.
  • Sterilize the media by autoclaving or filtration.
  • Adjust the pH and other parameters as necessary.

Constructing a Growth Curve:

  • Inoculate the organism in a suitable culture vessel (shake flask or bioreactor) with the appropriate media.
  • Measure the optical density or other growth parameters (e.g., dry weight, cell count, protein content) at regular intervals.
  • Plot the growth data over time to construct a growth curve.
  • Analyze the growth curve to determine the growth rate and other growth parameters.

Calculating Mass Transfer Coefficient in Fermentation:

  • The mass transfer coefficient is a measure of how efficiently oxygen is transferred from the air to the culture medium.
  • It can be calculated by measuring the oxygen transfer rate (OTR) and the volumetric oxygen transfer coefficient (kLa).
  • The OTR can be measured using an oxygen probe and the kLa can be determined experimentally by varying the agitation rate and/or aeration rate.
  • The mass transfer coefficient (kLa) can then be calculated using the following equation:
    kLa = OTR / (P_O2,sat – P_O2)
    Where:

    1. OTR is the oxygen transfer rate
    2. P_O2,sat is the saturation concentration of oxygen in the medium
    3. P_O2 is the partial pressure of oxygen in the medium.

Hire Writer For Custom Assignment Assistance

Assignment Brief 2: Extract soluble enzyme from fungi or bacteria, utilize the enzyme in soluble or immobilized form and calculate the enzyme activity and kinetics.

Extracting Soluble Enzyme from Fungi or Bacteria:

  1. Choose a suitable microorganism that produces the desired enzyme.
  2. Grow the microorganism in a suitable medium that supports its growth and enzyme production.
  3. Harvest the microorganism at the appropriate stage of growth.
  4. Disrupt the cells to release the enzyme. This can be achieved by various methods such as sonication, homogenization, or enzymatic digestion.
  5. Centrifuge the lysate to remove cellular debris and collect the supernatant containing the soluble enzyme.
  6. Store the enzyme at an appropriate temperature and pH until further use.

Utilizing the Enzyme in Soluble or Immobilized Form:

  1. Determine the optimal pH and temperature for enzyme activity.
  2. Choose a suitable substrate and measure its concentration.
  3. Add the enzyme to the substrate solution and incubate for a specific period.
  4. Measure the product concentration at regular intervals using a spectrophotometer or other appropriate method.
  5. Repeat the experiment with varying enzyme concentrations to determine enzyme activity.
  6. Immobilize the enzyme on a suitable support material such as agarose beads, silica gel, or magnetic nanoparticles, using a suitable method such as covalent bonding, adsorption, or encapsulation.
  7. Measure the enzyme activity of the immobilized enzyme using the same procedure as for the soluble enzyme.

Calculating the Enzyme Activity and Kinetics:

  1. Calculate the initial reaction rate using the slope of the linear part of the product concentration vs. the time curve.
  2. Determine the Michaelis-Menten constant (Km) and maximum reaction rate (Vmax) using Lineweaver-Burk or other appropriate kinetic analysis methods.
  3. Determine the effect of pH and temperature on enzyme activity and calculate the activation energy using the Arrhenius equation.
  4. Determine the effect of substrate concentration on enzyme activity and calculate the Hill coefficient to determine the degree of cooperativity.
  5. Compare the properties of the soluble and immobilized enzymes and determine the advantages and disadvantages of each form.

Assignment Brief 3: Differentiate the mechanism involved in cross flow filtration and in freeze and spray drying processes and predict the possibility factors the affecting the processes.

Cross-flow filtration and freeze/spray drying are two different processes used in the separation and drying of various products. The mechanisms involved in these processes are different, as are the factors that affect their efficiency and outcome.

Cross-flow filtration is a process that uses a membrane to separate a mixture into its individual components based on size and molecular weight. In this process, the mixture is fed into the filtration system, where it is pumped through the membrane. The membrane is designed to allow the smaller particles to pass through while retaining the larger particles. The filtrate is collected on one side of the membrane, and the retained particles are collected on the other side. This process is used for various applications such as water purification, protein concentration, and blood plasma separation.

On the other hand, freeze and spray drying are two different methods used to remove moisture from a product. In freeze drying, the product is first frozen, and then the moisture is removed by sublimation under a vacuum. Spray drying, on the other hand, involves the spraying of a liquid mixture into a hot chamber where the liquid droplets are converted into dry particles by evaporation. Both methods are commonly used in the food, pharmaceutical, and chemical industries.

The factors that affect the efficiency and outcome of these processes are different. In cross-flow filtration, the membrane’s quality, size, and pore size can affect the separation efficiency. Other factors such as feed concentration, flow rate, and temperature can also impact the process. In freeze and spray drying, the product’s composition, particle size, and viscosity can affect the drying efficiency. Other factors such as drying temperature, air velocity, and residence time can also affect the process.

Pay & Get Instant Solution Of Assignmets and Essays By Malaysian Writers

Get genuine CBE661 BIOPROCESS ENGINEERING LAB assignment solutions that are both plagiarism-free and affordable!

If you’re looking to ensure the timely delivery of high-quality work and complete your CBE661 BIOPROCESS ENGINEERING LAB assignments with ease, then opting for Malaysia Assignment Help is the ideal solution. With our team of experienced assignment writers, we offer affordable and top-notch services that have been catering to student needs for a significant period.

At our assignment help website, we offer assistance with essay assignments to students who may be facing difficulties with their tasks. Our team of essay writing experts acknowledges the significance of producing authentic and innovative essays and thus provides tailored essay services to meet your specific requirements. Moreover, students can access online assistance for their dissertations. Our team of dissertation experts possesses vast experience and proficiency in delivering dissertation writing help across a broad range of topics.

We can assist with any assignment you require help with. Our team of proficient writers are specialists in their respective fields and can furnish you with top-notch aid to help you attain the grades you desire. Thus, if you find yourself pondering “is there someone who can do my assignment for me?” then rest assured, the answer is a definite yes! Connect with us now for assignment help and receive the support necessary to achieve the grades you merit.

Private and Confidential

Yours all information is private and confidential; it is not shared with any other party. So, no one will know that you have taken help for your Academic paper from us.

Online Exam & Assignment Writing Services
Malaysia Assignment Order Image

11770+ Orders Delivered

Malaysia Assignment Order Star Rating

4.9/5
5 Star Rating

Confidential & Secure

Group Assignment Help

Online Exam -Test & Quiz

Cheapest Price Quote

Diploma & Certificate Levels

Semester & FYP Papers

Summative & Individual

GBA & Reflective

Last Minute Assistance

Ask Your Homework Today!

We have over 1000 academic writers ready and waiting to help you achieve academic success

Sample Assignment Download

CSC530 Fundamentals Of Multimedia Computing Assignment Example Malaysia
The course is designed to provide an integrated foundation from the fundamental aspects of human communications systems, technologies involved, and their limitations. It also provides you with information on forms…
Computer & It
CEM583 Law For Construction UITM Assignment Sample Malaysia
CEM583 Law for Construction is a course offered by Universiti Teknologi MARA (UiTM) that aims to provide students with a comprehensive understanding of the legal framework that governs the construction…
Law

UP TO 15 % DISCOUNT

Instant Paper Writing Services by Native Malaysia Writers

Plagiarism Free Solutions
100% Original Work
24*7 Online Assistance
Native PhD Experts
Hire a Writer Now

Get Assistance for Assignments, online Exam, and Projects Writing