CEV404 Environmental Microbiology UITM Assignment Sample Malaysia

CEV404 Environmental Microbiology is a course offered by Universiti Teknologi MARA (UiTM)! This course is designed to provide students with a comprehensive understanding of the microbial world and their role in the environment. Microbes are ubiquitous and play critical roles in global biogeochemical cycles, including the cycling of nutrients, decomposition, and the production of greenhouse gases.

The course will cover topics such as microbial ecology, biogeochemistry, microbial diversity, and the role of microbes in environmental processes such as bioremediation and wastewater treatment. Students will also learn about the techniques used to study microbes in the environment, including culturing, molecular biology, and metagenomics.

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Explore assignments for CEV404 Environmental Microbiology course 

Here, we will describe some assignment briefs. These are:

Assignment Brief 1: Explain the basic principles of environmental microbiology.

Environmental microbiology is the study of microorganisms in their natural habitats, including soil, water, air, and living organisms. The basic principles of environmental microbiology involve understanding the interactions between microorganisms and their environment, as well as the role of microorganisms in biogeochemical cycles, nutrient cycling, and ecosystem functioning.

  1. Microbial Diversity: One of the fundamental principles of environmental microbiology is the study of microbial diversity. Microorganisms are present in all types of environments and play vital roles in ecosystem processes, including nutrient cycling, decomposition, and energy flow.
  2. Microbial Interactions: Microorganisms interact with each other and with other organisms in their environment. For example, microbial interactions with plants and animals can influence nutrient cycling and energy flow within ecosystems. Microorganisms can also interact with each other in a variety of ways, including symbiotic relationships, competition for resources, and predation.
  3. Biogeochemical Cycles: Microorganisms are critical components of biogeochemical cycles, including the carbon, nitrogen, and phosphorus cycles. These cycles involve the movement of essential nutrients through different environmental compartments, including soil, water, and air.
  4. Microbial Ecology: Microbial ecology is the study of the interactions between microorganisms and their environment. This includes understanding the distribution and abundance of microorganisms in different habitats and how they respond to environmental changes.
  5. Microbial Biotechnology: Environmental microbiology also plays an essential role in the development of biotechnologies that harness microorganisms to remediate environmental problems. For example, microorganisms can be used to degrade pollutants, produce biofuels, and enhance crop productivity.

Overall, environmental microbiology is a multidisciplinary field that combines biology, ecology, chemistry, and engineering to understand the roles of microorganisms in ecosystem processes and develop innovative solutions to environmental challenges.

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Assignment Brief 2: Apply common microbiological techniques and skills in solving chemical/ environmental engineering problems.

Microbiology plays a crucial role in chemical and environmental engineering, as microbes are essential for various biotechnological processes. Here are some common microbiological techniques and skills that can be applied to solve chemical and environmental engineering problems:

  1. Sterilization: Sterilization is the process of killing or removing all living microorganisms from a particular material or environment. In chemical and environmental engineering, sterilization is used to prevent microbial contamination in bioreactors, laboratory equipment, and other materials used in microbial studies.
  2. Microbial culturing: Microbial culturing involves growing microorganisms in a controlled environment, such as a Petri dish, for further study. This technique is used to isolate and identify specific microorganisms and to study their growth and metabolism under different conditions.
  3. Microbial identification: Microbial identification involves characterizing and identifying microorganisms based on their morphological, physiological, and biochemical properties. This technique is essential in studying the microbial community and their role in bioremediation, wastewater treatment, and other environmental processes.
  4. Microbial growth kinetics: Microbial growth kinetics is the study of the growth rate and metabolism of microorganisms. This technique is used to optimize the growth conditions for microorganisms used in bioreactors and other biotechnological processes.
  5. DNA sequencing: DNA sequencing is used to identify and characterize microorganisms based on their genetic makeup. This technique is used in metagenomics studies to identify the microbial community in various environmental samples.
  6. Bioreactor design and operation: Bioreactors are used to grow and maintain microorganisms under controlled conditions. Understanding the principles of microbiology is essential in designing and operating bioreactors for various biotechnological processes.
  7. Microbial analysis of environmental samples: Microbial analysis of environmental samples involves isolating and identifying microorganisms from environmental samples such as soil, water, and air. This technique is used to study the microbial community in different environmental settings and their role in various environmental processes.

Assignment brief 3: Present the experimental findings related to environmental microbiology procedure and practice in a report and oral presentation.


Environmental microbiology is the study of microorganisms in their natural environment, including the soil, water, air, and living organisms. In this report, we will discuss the experimental findings related to environmental microbiology procedures and practices.

Materials and Methods:

For this study, we collected samples of soil and water from different locations. We used various techniques to isolate and identify the microorganisms present in the samples. The following techniques were used:

  1. Plating: We used agar plates with different media to isolate bacteria, fungi, and actinomycetes from the soil samples. The plates were incubated at different temperatures and durations, depending on the media and organisms being targeted.
  2. Microscopy: We used a compound light microscope to examine the morphology of the isolated microorganisms. We also used staining techniques such as Gram staining and acid-fast staining to differentiate between different types of bacteria.
  3. PCR: We used the polymerase chain reaction (PCR) to amplify specific genes in the DNA of the microorganisms. This allowed us to identify the species of the isolated microorganisms.


Our findings revealed the following:

  1. Diversity of microorganisms: We identified a diverse range of microorganisms in the soil and water samples, including bacteria, fungi, actinomycetes, and viruses.
  2. Abundance of bacteria: Bacteria were the most abundant microorganisms in the soil samples, with up to 10^9 CFU/g of soil.
  3. Differentiation of bacteria: The use of staining techniques such as Gram staining allowed us to differentiate between Gram-positive and Gram-negative bacteria. We also identified acid-fast bacteria using the acid-fast staining technique.
  4. Identification of microorganisms: PCR allowed us to identify the species of the isolated microorganisms. We identified various species of bacteria, fungi, and actinomycetes.
  5. Antibiotic resistance: We found that some of the bacteria isolated from the soil samples were resistant to antibiotics such as penicillin, tetracycline, and erythromycin.


In conclusion, our experimental findings demonstrate the importance of environmental microbiology in identifying and understanding the microorganisms present in our environment. The use of different techniques such as plating, microscopy, and PCR allowed us to isolate and identify a diverse range of microorganisms in soil and water samples. Our findings also revealed the presence of antibiotic-resistant bacteria in the environment, highlighting the need for further research and action to address this issue.

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