CBE552 Genetic Engineering UITM Assignment Sample Malaysia

CBE552 Genetic Engineering course is an undergraduate course offered by UITM. The course is an introduction to the fundamental principles of genetic engineering. The course covers topics such as DNA structure and function, RNA structure and function, protein structure and function, and genetic recombination. The course also covers the ethical and social implications of genetic engineering.

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In this segment, we provide a few assignment activities. These are:

Assignment Activity 1: Demonstrate an understanding of the principles of genetic engineering, biotechnology and recombinant DNA technology.

Genetic engineering, biotechnology, and recombinant DNA technology are branches of science that involve manipulating the genetic material of organisms for various purposes. Here is a brief overview of the principles of these three areas:

Genetic Engineering:

Genetic engineering is the process of manipulating the genetic material of an organism by introducing or altering specific genes. This process can be used to improve or modify an organism’s traits, create new traits, or produce desired products. Genetic engineering is commonly used in agriculture, medicine, and industry. For example, genetically modified crops are designed to be more resistant to pests or herbicides, while gene therapy is used to treat genetic disorders by replacing or repairing a patient’s faulty genes.

Biotechnology:

Biotechnology involves the use of living organisms or parts of organisms to produce useful products or processes. This can include the use of microbes, plants, or animals to produce medicines, food, or industrial chemicals. Biotechnology also involves the development of new technologies to study and manipulate biological systems. For example, scientists can use biotechnology to develop new drugs or vaccines, improve agricultural productivity, or create new biofuels.

Recombinant DNA technology:

Recombinant DNA technology involves the manipulation of DNA molecules to create new combinations of genes that do not occur naturally. This technology allows scientists to combine genes from different organisms to create new traits or products. Recombinant DNA technology is used in genetic engineering and biotechnology, as well as in medical research to create new treatments for diseases. For example, insulin used to treat diabetes is produced using recombinant DNA technology, where a human insulin gene is inserted into bacteria, which then produces the insulin for use in patients.

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Assignment Activity 2: Show a basic level of competency in problem solving, data processing and analysis associated with the field of genetic engineering.

Genetic engineering is a branch of biotechnology that involves modifying the genetic material of organisms to achieve desired traits. The process involves identifying a specific genetic sequence, isolating it, and manipulating it using various techniques. The modified genetic material is then introduced into the organism, where it is expressed, leading to the desired trait.

Problem-solving in genetic engineering involves identifying the desired trait or characteristic and the specific genetic sequence responsible for it. Scientists use various methods, such as gene mapping and DNA sequencing, to identify the target sequence. Once the target sequence is identified, scientists design experiments to manipulate the sequence and introduce it into the target organism.

Data processing and analysis in genetic engineering involve using various software tools and statistical methods to analyze genetic data. Scientists use various software tools, such as BLAST, GeneMark, and ClustalW, to analyze DNA and protein sequences. Statistical methods, such as chi-squared tests and ANOVA, are used to analyze experimental data and determine the significance of the results.

Assignment Activity 3: Utilise and provide information on techniques used to manipulate living cells and overview genetic engineering methods.

Techniques for Manipulating Living Cells:

  1. Transfection: Transfection is a technique for introducing foreign DNA into a cell, often used for studying gene expression and protein function. The DNA can be inserted using a variety of methods, such as electroporation, lipofection, or viral vectors.
  2. Microinjection: Microinjection is a technique in which a tiny needle is used to inject DNA or other substances directly into a cell. This technique is often used for introducing DNA into fertilized eggs or for introducing specific molecules or proteins into a cell.
  3. CRISPR/Cas9: CRISPR/Cas9 is a revolutionary gene editing technique that allows researchers to make precise changes to the DNA of living cells. The technique uses a molecular tool that targets specific regions of DNA and cuts them, enabling researchers to add, delete, or replace specific genes.
  4. Cloning: Cloning involves creating a genetically identical copy of a cell, organism, or DNA fragment. This technique can be used to study gene function or to create large quantities of a specific protein or other substance.

Overview of Genetic Engineering Methods:

  1. Restriction Enzyme Digestion: Restriction enzymes are molecular scissors that cut DNA at specific sites. This technique is often used to create fragments of DNA that can be used in other genetic engineering methods.
  2. Polymerase Chain Reaction (PCR): PCR is a technique used to amplify small amounts of DNA into larger quantities. This technique is often used in genetic engineering to create large quantities of a specific DNA fragment for use in other methods.
  3. Gene Synthesis: Gene synthesis involves creating a specific DNA sequence using chemical methods. This technique is often used to create custom genes for use in genetic engineering.
  4. Gene Editing: Gene editing involves making specific changes to the DNA sequence of an organism. This can be done using a variety of techniques, such as CRISPR/Cas9, TALENs, or ZFNs.
  5. Transgenic Technology: Transgenic technology involves inserting foreign DNA into the genome of an organism. This can be done using a variety of methods, such as transfection or microinjection. The resulting organisms are known as transgenic organisms and can be used to study gene function or to create genetically modified crops or animals.

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