Enzim E memangkin hidrolisis substrat S Kinetik E (V0) sebagai satu fungsi S diukur dan data diperoleh seperti dalam Jadual: Environmental Science Assignment, UON, Malaysia

University University of Nottingham (UON)
Subject Environmental Science

1. Enzim E memangkin hidrolisis substrat S. Kinetik E (V0) sebagai satu fungsi S diukur dan data diperoleh seperti dalam Jadual 1. Enzim mematuhi kinetik
Michaelis-Menten, yang diberi melalui persamaan berikut: [The enzyme E catalyzes the hydrolysis of substrate S. The kinetics of E (V0) as a function of S were measured and the data were obtained as in Table 1. The enzyme obeys Michaelis-Menten kinetics, which is given by the following equation:]

yang mana Vmax ialah kadar maksimum tindakbalas dan KM ialah pemalar
Michaelis-Menten. [where Vmax is the maximum reaction rate and KM is the Michaelis-Menten constant.]

a) Plot data menggunakan angka salingan berganda (Lineweaver-Burk).
[Plot the data using the double reciprocal (Lineweaver-Burk).]
b) Tentukan Vmax dan KM daripada graf anda.

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